Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

MLKL ELISA Kit

MLKL Reactivity: Human, Mouse pSer345 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Catalog No. ABIN6810519
  • Target See all MLKL ELISA Kits
    MLKL (Mixed Lineage Kinase Domain-Like (MLKL))
    Binding Specificity
    pSer345
    Reactivity
    Human, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human and Mouse Phospho-MLKL (Ser345) ELISA Kit. This assay semi-quantitatively measures MLKL phosphorylated at Serine-345 in cell lysate samples.
    Sample Type
    Cell Samples, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human and Mouse MLKL phosphorylated at site Serine-345.
    Characteristics
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product MLKL ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all MLKL ELISA Kits
    MLKL (Mixed Lineage Kinase Domain-Like (MLKL))
    Alternative Name
    MLKL (MLKL Products)
    Synonyms
    MLKL ELISA Kit, 9130019I15Rik ELISA Kit, mixed lineage kinase domain like pseudokinase ELISA Kit, mixed lineage kinase domain-like ELISA Kit, MLKL ELISA Kit, mlkl ELISA Kit, Mlkl ELISA Kit
    Gene ID
    197259
    UniProt
    Q8NB16, Q9D2Y4
You are here:
Support