SAE1 ELISA Kit

Catalog No. ABIN6951062

Product Details SAE1 ELISA Kit

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SAE1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1 (SAE1 Products)

Synonyms: AOS1 ELISA Kit, HSPC140 ELISA Kit, SUA1 ELISA Kit, UBLE1A ELISA Kit, 2400010M20Rik ELISA Kit, 2610044L12Rik ELISA Kit, AL033372 ELISA Kit, AW743391 ELISA Kit, D7Ertd177e ELISA Kit, Sua1 ELISA Kit, Uble1a ELISA Kit, Aos1p ELISA Kit, Sua1p ELISA Kit, aos ELISA Kit, sae2a ELISA Kit, uble1a ELISA Kit, wu:fa28b04 ELISA Kit, zgc:86633 ELISA Kit, SUMO1 activating enzyme subunit 1 ELISA Kit, SUMO1 activating enzyme subunit 1 L homeolog ELISA Kit, SAE1 ELISA Kit, Sae1 ELISA Kit, sae1.L ELISA Kit, sae1 ELISA Kit

Gene ID: 10055

UniProt: Q9UBE0