Amphiphysin ELISA Kit

Catalog No. ABIN6951072

Product Details Amphiphysin ELISA Kit

Target: Amphiphysin (AMPH)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human AMPH ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Amphiphysin

Target: Amphiphysin (AMPH)

Alternative Name: AMPH (AMPH Products)

Synonyms: Amp ELISA Kit, CG8604 ELISA Kit, DAMP ELISA Kit, Damp ELISA Kit, Dmel\\CG8604 ELISA Kit, amph ELISA Kit, dAmph ELISA Kit, damph ELISA Kit, AMPH ELISA Kit, Amph ELISA Kit, GB16263 ELISA Kit, AMPH1 ELISA Kit, Amph1 ELISA Kit, wu:fq25h04 ELISA Kit, zgc:73193 ELISA Kit, amphiphysin ELISA Kit, Amphiphysin ELISA Kit, amphiphysin L homeolog ELISA Kit, AMPH ELISA Kit, Amph ELISA Kit, amph ELISA Kit, LOC409851 ELISA Kit, amph.L ELISA Kit, LOC100343468 ELISA Kit

Gene ID: 273

UniProt: P49418