DARS ELISA Kit

Catalog No. ABIN6951073

Product Details DARS ELISA Kit

Target: DARS (Aspartyl-tRNA Synthetase (DARS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human DARS ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for DARS

Target: DARS (Aspartyl-tRNA Synthetase (DARS))

Alternative Name: DARS (DARS Products)

Synonyms: dars ELISA Kit, MGC53970 ELISA Kit, MGC76305 ELISA Kit, DARS ELISA Kit, Aats-Asp ELISA Kit, AspRS ELISA Kit, CG3821 ELISA Kit, DRS ELISA Kit, Dmel\\CG3821 ELISA Kit, aats-asp ELISA Kit, anon-EST:Liang-2.49 ELISA Kit, cDNA1 ELISA Kit, clone 2.49 ELISA Kit, l(2)49Db ELISA Kit, l(2)k04508 ELISA Kit, l(2)v27 ELISA Kit, l(2)vr1 ELISA Kit, poney ELISA Kit, vr1 ELISA Kit, MGC80207 ELISA Kit, wu:fc17a11 ELISA Kit, zgc:154056 ELISA Kit, ECK1867 ELISA Kit, JW1855 ELISA Kit, tls ELISA Kit, HBSL ELISA Kit, 5730439G15Rik ELISA Kit, DRS1 ELISA Kit, aspartyl-tRNA synthetase S homeolog ELISA Kit, aspartyl-tRNA synthetase ELISA Kit, Aspartyl-tRNA synthetase ELISA Kit, aspartyl-tRNA synthetase L homeolog ELISA Kit, aspartate--tRNA(Asn) ligase ELISA Kit, Aspartate--tRNA ligase, cytoplasmic ELISA Kit, dars.S ELISA Kit, dars ELISA Kit, DARS ELISA Kit, AspRS ELISA Kit, dars.L ELISA Kit, aspS ELISA Kit, RMDY18_09660 ELISA Kit, Tbis_2053 ELISA Kit, Slip_0775 ELISA Kit, Aats-asp ELISA Kit, MFER_RS02995 ELISA Kit, CKC_01935 ELISA Kit, Dars ELISA Kit, dars-1 ELISA Kit

Gene ID: 1615

UniProt: P14868