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PBK ELISA Kit

PBK Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951206
  • Target See all PBK ELISA Kits
    PBK (PDZ Binding Kinase (PBK))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human PBK ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product PBK ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all PBK ELISA Kits
    PBK (PDZ Binding Kinase (PBK))
    Alternative Name
    PBK (PBK Products)
    Synonyms
    PBK ELISA Kit, topk ELISA Kit, CT84 ELISA Kit, Nori-3 ELISA Kit, SPK ELISA Kit, TOPK ELISA Kit, 2810434B10Rik ELISA Kit, AW538537 ELISA Kit, D14Ertd732e ELISA Kit, zgc:92050 ELISA Kit, PDZ binding kinase ELISA Kit, PDZ binding kinase L homeolog ELISA Kit, PBK ELISA Kit, pbk ELISA Kit, Pbk ELISA Kit, pbk.L ELISA Kit
    Gene ID
    55872
    UniProt
    Q96KB5
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