VLDLR ELISA Kit
VLDLR
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all VLDLR ELISA Kits
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VLDLR
(Very Low Density Lipoprotein Receptor (VLDLR))
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human VLDL-R ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Application Notes
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Optimal working dilution should be determined by the investigator.
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Expiry Date
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6 months
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Target
See all VLDLR ELISA Kits
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VLDLR
(Very Low Density Lipoprotein Receptor (VLDLR))
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Alternative Name
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VLDL-R (VLDLR Products)
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Synonyms
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CARMQ1 ELISA Kit, CHRMQ1 ELISA Kit, VLDLRCH ELISA Kit, AA408956 ELISA Kit, AI451093 ELISA Kit, AW047288 ELISA Kit, vldlr ELISA Kit, very low density lipoprotein receptor ELISA Kit, very low density lipoprotein receptor S homeolog ELISA Kit, VLDLR ELISA Kit, Vldlr ELISA Kit, vldlr.S ELISA Kit
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Gene ID
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7436
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UniProt
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P98155
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Pathways
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Cellular Response to Molecule of Bacterial Origin
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