RRM1 ELISA Kit

Catalog No. ABIN6951351

Product Details RRM1 ELISA Kit

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human RRM1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for RRM1

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Alternative Name: RRM1 (RRM1 Products)

Synonyms: CG5371 ELISA Kit, DmRNR1 ELISA Kit, Dmel\\CG5371 ELISA Kit, RNR1 ELISA Kit, RnrM1 ELISA Kit, RntL ELISA Kit, l(2)k06709 ELISA Kit, XRRM1 ELISA Kit, R1 ELISA Kit, RIR1 ELISA Kit, RR1 ELISA Kit, CHUNP6866 ELISA Kit, cb396 ELISA Kit, cb838 ELISA Kit, r1 ELISA Kit, sb:cb548 ELISA Kit, wu:fb39b07 ELISA Kit, wu:fi14b02 ELISA Kit, wu:fk95f07 ELISA Kit, ribonucleotide reductase catalytic subunit M1 ELISA Kit, Ribonucleoside diphosphate reductase large subunit ELISA Kit, ribonucleoside-diphosphate reductase large subunit ELISA Kit, ribonucleotide reductase M1 L homeolog ELISA Kit, ribonucleotide reductase M1 ELISA Kit, ribonucleotide reductase M1 polypeptide ELISA Kit, RRM1 ELISA Kit, RnrL ELISA Kit, LOC100472317 ELISA Kit, rrm1 ELISA Kit, rrm1.L ELISA Kit, Rrm1 ELISA Kit

Gene ID: 6240

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity