EIF5A2 ELISA Kit

Catalog No. ABIN6951365

Product Details EIF5A2 ELISA Kit

Target: EIF5A2 (Eukaryotic Translation Initiation Factor 5A2 (EIF5A2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human EIF5A2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for EIF5A2

Target: EIF5A2 (Eukaryotic Translation Initiation Factor 5A2 (EIF5A2))

Alternative Name: EIF5A2 (EIF5A2 Products)

Synonyms: EIF5A2 ELISA Kit, EIF5A ELISA Kit, zgc:55504 ELISA Kit, zgc:77429 ELISA Kit, wu:fb05b06 ELISA Kit, wu:fc23f04 ELISA Kit, wu:fc61c10 ELISA Kit, EIF-5A2 ELISA Kit, eIF5AII ELISA Kit, 9630038B20 ELISA Kit, eukaryotic translation initiation factor 5A2 ELISA Kit, eukaryotic translation initiation factor 5A3 ELISA Kit, EIF5A2 ELISA Kit, eif5a2 ELISA Kit, LOC100499632 ELISA Kit, Eif5a2 ELISA Kit

Gene ID: 56648

UniProt: Q9GZV4