NDRG1 ELISA Kit

Catalog No. ABIN6951436

Product Details NDRG1 ELISA Kit

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human NDRG1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for NDRG1

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Alternative Name: NDRG1 (NDRG1 Products)

Synonyms: CAP43 ELISA Kit, CMT4D ELISA Kit, DRG-1 ELISA Kit, DRG1 ELISA Kit, GC4 ELISA Kit, HMSNL ELISA Kit, NDR1 ELISA Kit, NMSL ELISA Kit, PROXY1 ELISA Kit, RIT42 ELISA Kit, RTP ELISA Kit, TARG1 ELISA Kit, TDD5 ELISA Kit, Ndr1 ELISA Kit, Ndrl ELISA Kit, cap43 ELISA Kit, cmt4d ELISA Kit, drg1 ELISA Kit, gc4 ELISA Kit, hmsnl ELISA Kit, ndr1 ELISA Kit, ndrg1 ELISA Kit, nmsl ELISA Kit, proxy1 ELISA Kit, rit42 ELISA Kit, rtp ELISA Kit, targ1 ELISA Kit, tdd5 ELISA Kit, xndrg1 ELISA Kit, ndrg4 ELISA Kit, cb775 ELISA Kit, wu:fb60h02 ELISA Kit, zgc:63944 ELISA Kit, NDRG1 ELISA Kit, NON-RACE SPECIFIC DISEASE RESISTANCE PROTEIN ELISA Kit, non race-specific disease resistance 1 ELISA Kit, ndrg1-a ELISA Kit, ndrg1-b ELISA Kit, xNDRG1-A ELISA Kit, ndrg1l ELISA Kit, zgc:73047 ELISA Kit, N-myc downstream regulated 1 ELISA Kit, N-myc downstream regulated gene 1 ELISA Kit, N-myc downstream regulated 1 S homeolog ELISA Kit, N-myc downstream regulated 1a ELISA Kit, Late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein family ELISA Kit, N-myc downstream regulated 1 L homeolog ELISA Kit, N-myc downstream regulated 1b ELISA Kit, NDRG1 ELISA Kit, Ndrg1 ELISA Kit, ndrg1.S ELISA Kit, ndrg1 ELISA Kit, ndrg1a ELISA Kit, NDR1 ELISA Kit, ndrg1.L ELISA Kit, ndrg1b ELISA Kit

Gene ID: 10397

UniProt: Q92597