DHRS9 ELISA Kit

Catalog No. ABIN6951437

Product Details DHRS9 ELISA Kit

Target: DHRS9 (Dehydrogenase/reductase (SDR Family) Member 9 (DHRS9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human DHRS9 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for DHRS9

Target: DHRS9 (Dehydrogenase/reductase (SDR Family) Member 9 (DHRS9))

Alternative Name: DHRS9 (DHRS9 Products)

Synonyms: 3ALPHA-HSD ELISA Kit, RDH-TBE ELISA Kit, RDH15 ELISA Kit, RDHL ELISA Kit, RDHTBE ELISA Kit, RETSDR8 ELISA Kit, SDR9C4 ELISA Kit, Rdhl ELISA Kit, C730025I08Rik ELISA Kit, Rdh15 ELISA Kit, rdhl ELISA Kit, 3alpha-hsd ELISA Kit, rdh15 ELISA Kit, retsdr8 ELISA Kit, DHRS9 ELISA Kit, RDHA ELISA Kit, rdh1l ELISA Kit, wu:fb64b07 ELISA Kit, zgc:73286 ELISA Kit, dehydrogenase/reductase 9 ELISA Kit, dehydrogenase/reductase (SDR family) member 9 ELISA Kit, dehydrogenase/reductase (SDR family) member 9 L homeolog ELISA Kit, DHRS9 ELISA Kit, Dhrs9 ELISA Kit, dhrs9.L ELISA Kit, dhrs9 ELISA Kit

Gene ID: 10170

UniProt: Q9BPW9

Pathways: C21-Steroid Hormone Metabolic Process