GPD1 ELISA Kit

Catalog No. ABIN6951445

Product Details GPD1 ELISA Kit

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GPD1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for GPD1

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Alternative Name: GPD1 (GPD1 Products)

Synonyms: GPDH ELISA Kit, Gpd3 ELISA Kit, GPD-C ELISA Kit, GPDH-C ELISA Kit, HTGTI ELISA Kit, CG9042 ELISA Kit, DROGPDHA ELISA Kit, DmG3PDH ELISA Kit, Dmel\\CG9042 ELISA Kit, G3PDH ELISA Kit, G3pdh ELISA Kit, GAPDH ELISA Kit, GPD ELISA Kit, GPDA ELISA Kit, GPDH-1 ELISA Kit, Gdh ELISA Kit, Gpd ELISA Kit, alpha-GPD ELISA Kit, alpha-GPDH ELISA Kit, alpha-GPDH-1 ELISA Kit, alpha-Gpdh ELISA Kit, alphaGPDH ELISA Kit, alphaGpd ELISA Kit, alphaGpdh ELISA Kit, alphaGpdh-1 ELISA Kit, gpdh ELISA Kit, gpdh-1 ELISA Kit, sn-Gpdh ELISA Kit, AI747587 ELISA Kit, Gdc-1 ELISA Kit, Gdc1 ELISA Kit, mKIAA4010 ELISA Kit, gpd1 ELISA Kit, wu:fc30a07 ELISA Kit, zgc:63859 ELISA Kit, MGC79676 ELISA Kit, GPD1 ELISA Kit, LG3P ELISA Kit, zgc:112197 ELISA Kit, Gpd1 ELISA Kit, gpd1h ELISA Kit, gpd1l ELISA Kit, wu:fc58b05 ELISA Kit, zgc:66051 ELISA Kit, zgc:85742 ELISA Kit, glycerol-3-phosphate dehydrogenase 1 ELISA Kit, Glycerol-3-phosphate dehydrogenase [NAD(+)] ELISA Kit, glycerol-3-phosphate dehydrogenase ELISA Kit, Glycerol 3 phosphate dehydrogenase ELISA Kit, glycerol-3-phosphate dehydrogenase 1 (soluble) ELISA Kit, glycerol-3-phosphate dehydrogenase 1 L homeolog ELISA Kit, glycerol-3-phosphate dehydrogenase 1c ELISA Kit, glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic ELISA Kit, glycerol-3-phosphate dehydrogenase 1a ELISA Kit, glycerol-3-phosphate dehydrogenase 1b ELISA Kit, Gpd1 ELISA Kit, gpdh-1 ELISA Kit, GPD1 ELISA Kit, Tb11.02.5280 ELISA Kit, Gpdh ELISA Kit, gpd1.L ELISA Kit, gpd1c ELISA Kit, gpd1 ELISA Kit, LOC101071719 ELISA Kit, gpd1a ELISA Kit, gpd1b ELISA Kit

Gene ID: 2819

UniProt: P21695