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Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) ELISA Kit

CMPK1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951480
  • Target See all Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) ELISA Kits
    Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)
    Reactivity
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human CMPK1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) ELISA Kits
    Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)
    Alternative Name
    CMPK1 (CMPK1 Products)
    Synonyms
    CK ELISA Kit, CMK ELISA Kit, CMPK ELISA Kit, UMK ELISA Kit, UMP-CMPK ELISA Kit, UMPK ELISA Kit, UMP/CMPK ELISA Kit, PSPTO1749 ELISA Kit, cmpk1 ELISA Kit, fb52d08 ELISA Kit, fk47a07 ELISA Kit, ump ELISA Kit, wu:fb52d08 ELISA Kit, wu:fk47a07 ELISA Kit, zgc:56452 ELISA Kit, Cmpk ELISA Kit, RGD1310116 ELISA Kit, cmpk ELISA Kit, 0610011D08Rik ELISA Kit, UMP-CMPk ELISA Kit, UCK ELISA Kit, cytidine/uridine monophosphate kinase 1 ELISA Kit, cytidylate kinase ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic S homeolog ELISA Kit, cytidylate kinase Cmk ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1 ELISA Kit, CMPK1 ELISA Kit, cmk ELISA Kit, cmpk1 ELISA Kit, cmpk ELISA Kit, Cmpk1 ELISA Kit, cmpk1.S ELISA Kit, Mrub_0478 ELISA Kit, MMAH_RS06670 ELISA Kit, TAGG_RS04320 ELISA Kit, Trad_0784 ELISA Kit, Deba_2713 ELISA Kit, Toce_1154 ELISA Kit, VDIS_RS03585 ELISA Kit, Fbal_1939 ELISA Kit, Ilyop_1379 ELISA Kit, Lbys_3481 ELISA Kit, Calni_0093 ELISA Kit, Ftrac_1556 ELISA Kit, Ocepr_1777 ELISA Kit
    Gene ID
    51727
    UniProt
    P30085
    Pathways
    Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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