PHAP1 ELISA Kit

Catalog No. ABIN6951526

Product Details PHAP1 ELISA Kit

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ANP32A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for PHAP1

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Alternative Name: ANP32A (ANP32A Products)

Synonyms: C15orf1 ELISA Kit, HPPCn ELISA Kit, I1PP2A ELISA Kit, LANP ELISA Kit, MAPM ELISA Kit, PHAP1 ELISA Kit, PHAPI ELISA Kit, PP32 ELISA Kit, Anp32 ELISA Kit, W91701 ELISA Kit, pp32 ELISA Kit, Anp32a ELISA Kit, CG5784 ELISA Kit, CT18148 ELISA Kit, CT42180 ELISA Kit, Dmel\\CG5784 ELISA Kit, anon-EST:fe3A2 ELISA Kit, wu:fj36e08 ELISA Kit, zgc:55516 ELISA Kit, ANP32D ELISA Kit, acidic nuclear phosphoprotein 32 family member A ELISA Kit, acidic (leucine-rich) nuclear phosphoprotein 32 family, member A ELISA Kit, CG5784 gene product from transcript CG5784-RB ELISA Kit, acidic nuclear phosphoprotein 32 family member A L homeolog ELISA Kit, ANP32A ELISA Kit, Anp32a ELISA Kit, Mapmodulin ELISA Kit, anp32a ELISA Kit, anp32a.L ELISA Kit

Gene ID: 8125

UniProt: P39687