IP6K1 ELISA Kit

Catalog No. ABIN6951627

Product Details IP6K1 ELISA Kit

Target: IP6K1 (Inositol Hexakisphosphate Kinase 1 (IP6K1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human IP6K1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for IP6K1

Target: IP6K1 (Inositol Hexakisphosphate Kinase 1 (IP6K1))

Alternative Name: IP6K1 (IP6K1 Products)

Synonyms: ihpk1 ELISA Kit, MGC80337 ELISA Kit, IP6K1 ELISA Kit, IHPK1 ELISA Kit, pius ELISA Kit, DKFZp459D1030 ELISA Kit, Ihpk1 ELISA Kit, Itpk6 ELISA Kit, PiUS ELISA Kit, 1200016D08Rik ELISA Kit, AU022776 ELISA Kit, InsP6 ELISA Kit, InsP6k1 ELISA Kit, inositol hexaphosphate kinase 1 L homeolog ELISA Kit, inositol hexakisphosphate kinase 1 ELISA Kit, inositol hexaphosphate kinase 1 ELISA Kit, ip6k1.L ELISA Kit, IP6K1 ELISA Kit, ip6k1 ELISA Kit, Tsp_08677 ELISA Kit, Ip6k1 ELISA Kit

Gene ID: 9807

UniProt: Q92551