GGT1 ELISA Kit

Catalog No. ABIN6951629

Product Details GGT1 ELISA Kit

Target: GGT1 (gamma Glutamyltransferase 1 (GGT1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Rat GGT1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for GGT1

Target: GGT1 (gamma Glutamyltransferase 1 (GGT1))

Alternative Name: GGT1 (GGT1 Products)

Synonyms: ggt1 ELISA Kit, zgc:64172 ELISA Kit, CD224 ELISA Kit, D22S672 ELISA Kit, D22S732 ELISA Kit, GGT ELISA Kit, GGT 1 ELISA Kit, GTG ELISA Kit, GGT-1 ELISA Kit, Ggtp ELISA Kit, dwg ELISA Kit, GGLUT ELISA Kit, Ggt ELISA Kit, GGT2 ELISA Kit, cd224 ELISA Kit, ggt ELISA Kit, gtg ELISA Kit, GGT1 ELISA Kit, ggt1-a ELISA Kit, gamma-glutamyltransferase 1a ELISA Kit, gamma-glutamyltransferase 1 ELISA Kit, gamma-glutamyltranspeptidase 1 ELISA Kit, gamma-glutamyltransferase 1 L homeolog ELISA Kit, gamma-glutamyltransferase ELISA Kit, gamma-glutamyltranspeptidase Ggt1 ELISA Kit, ggt1a ELISA Kit, GGT1 ELISA Kit, Ggt1 ELISA Kit, ggt1 ELISA Kit, LOC470168 ELISA Kit, ggt1.L ELISA Kit, ggt ELISA Kit, Bcep18194_A6223 ELISA Kit, h16_A1098 ELISA Kit, Shewana3_2487 ELISA Kit, Bcep1808_2978 ELISA Kit

Gene ID: 116568

UniProt: P07314