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PFN2 ELISA Kit

PFN2 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951682
  • Target See all PFN2 ELISA Kits
    PFN2 (Profilin 2 (PFN2))
    Reactivity
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human PFN2 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product PFN2 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all PFN2 ELISA Kits
    PFN2 (Profilin 2 (PFN2))
    Alternative Name
    PFN2 (PFN2 Products)
    Synonyms
    D3S1319E ELISA Kit, PFL ELISA Kit, Pfn ELISA Kit, Profilin2 ELISA Kit, XProfilin2 ELISA Kit, PFN2 ELISA Kit, PRO2 ELISA Kit, Profilin-2 ELISA Kit, pfl ELISA Kit, d3s1319e ELISA Kit, profilin-2 ELISA Kit, F17A13.170 ELISA Kit, F17A13_170 ELISA Kit, PRF2 ELISA Kit, PROFILIN ELISA Kit, PROFILIN 2 ELISA Kit, profilin 2 ELISA Kit, wu:fb01g08 ELISA Kit, wu:fb54f09 ELISA Kit, wu:fd58f06 ELISA Kit, profilin 2 ELISA Kit, profilin 2 L homeolog ELISA Kit, profilin homolog 2 ELISA Kit, Profilin-2 ELISA Kit, PFN2 ELISA Kit, Pfn2 ELISA Kit, pfn2.L ELISA Kit, prf2 ELISA Kit, pfn2 ELISA Kit, pfn-2 ELISA Kit
    Gene ID
    5217
    UniProt
    P35080
    Pathways
    Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
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