RUVBL1 ELISA Kit

Catalog No. ABIN6951691

Product Details RUVBL1 ELISA Kit

Target: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human RUVBL1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for RUVBL1

Target: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Alternative Name: RUVBL1 (RUVBL1 Products)

Synonyms: rvb1 ELISA Kit, ecp54 ELISA Kit, tip49 ELISA Kit, nmp238 ELISA Kit, pontin ELISA Kit, tip49a ELISA Kit, xpontin ELISA Kit, pontin52 ELISA Kit, TIP49 ELISA Kit, wu:fd18e04 ELISA Kit, zpontin ELISA Kit, ECP54 ELISA Kit, INO80H ELISA Kit, NMP238 ELISA Kit, PONTIN ELISA Kit, Pontin52 ELISA Kit, RVB1 ELISA Kit, TIH1 ELISA Kit, TIP49A ELISA Kit, Tip49a ELISA Kit, 2510009G06Rik ELISA Kit, RuvB like AAA ATPase 1 ELISA Kit, RuvB-like 1 (E. coli) ELISA Kit, RuvB-like 1 ELISA Kit, RuvB like AAA ATPase 1 S homeolog ELISA Kit, RuvB-like AAA ATPase 1 ELISA Kit, RuvB-like protein 1 ELISA Kit, RUVBL1 ELISA Kit, ruvbl1 ELISA Kit, PVX_123295 ELISA Kit, Bm1_15605 ELISA Kit, ruvb1 ELISA Kit, ruvbl1.S ELISA Kit, Ruvbl1 ELISA Kit

Gene ID: 8607

UniProt: Q9Y265

Pathways: Telomere Maintenance