Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RNASE1 ELISA Kit

RNASE1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951694
  • Target See all RNASE1 ELISA Kits
    RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
    Reactivity
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human RNASE1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product RNASE1 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all RNASE1 ELISA Kits
    RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
    Alternative Name
    RNASE1 (RNASE1 Products)
    Synonyms
    RNASE1 ELISA Kit, RNS1 ELISA Kit, ATRNS1 ELISA Kit, RIBONUCLEASE 1 ELISA Kit, T17M13.16 ELISA Kit, T17M13_16 ELISA Kit, ribonuclease 1 ELISA Kit, RIB1 ELISA Kit, AI574248 ELISA Kit, Rib-1 ELISA Kit, Rib1 ELISA Kit, SRN ELISA Kit, ribonuclease A family member 1, pancreatic ELISA Kit, ribonuclease pancreatic-like ELISA Kit, ribonuclease A family member k6 ELISA Kit, Ribonuclease 1 ELISA Kit, ribonuclease 1 ELISA Kit, ribonuclease, RNase A family, 1 (pancreatic) ELISA Kit, ribonuclease pancreatic ELISA Kit, RNASE1 ELISA Kit, LOC475395 ELISA Kit, RNASE6 ELISA Kit, Rnase1 ELISA Kit, RNS1 ELISA Kit, LOC101113761 ELISA Kit
    Gene ID
    6035
    UniProt
    P07998
You are here:
Support