SRPK1 ELISA Kit

Catalog No. ABIN6951698

Product Details SRPK1 ELISA Kit

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SRPK1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for SRPK1

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Alternative Name: SRPK1 (SRPK1 Products)

Synonyms: SFRSK1 ELISA Kit, AU017960 ELISA Kit, BEST:CK01209 ELISA Kit, CG11489 ELISA Kit, CG9085 ELISA Kit, CK01209 ELISA Kit, Dmel\\CG11489 ELISA Kit, SRPK ELISA Kit, SRPK1 ELISA Kit, Srpk79D ELISA Kit, atc ELISA Kit, srpk1 ELISA Kit, zgc:63789 ELISA Kit, wu:fc06h03 ELISA Kit, zgc:158800 ELISA Kit, sfrsk1 ELISA Kit, SRSF protein kinase 1 ELISA Kit, serine/arginine-rich protein specific kinase 1 ELISA Kit, Serine-arginine protein kinase at 79D ELISA Kit, SRSF protein kinase 1a ELISA Kit, SRSF protein kinase 1b ELISA Kit, SRSF protein kinase 1 S homeolog ELISA Kit, SRPK1 ELISA Kit, Srpk1 ELISA Kit, Srpk79D ELISA Kit, srpk1a ELISA Kit, srpk1b ELISA Kit, srpk1.S ELISA Kit

Gene ID: 6732

UniProt: Q96SB4

Pathways: Toll-Like Receptors Cascades