GFPT1 ELISA Kit

Catalog No. ABIN6951707

Product Details GFPT1 ELISA Kit

Target: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GFPT1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for GFPT1

Target: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Alternative Name: GFPT1 (GFPT1 Products)

Synonyms: gfat ELISA Kit, 2810423A18Rik ELISA Kit, AI324119 ELISA Kit, AI449986 ELISA Kit, GFA ELISA Kit, GFAT ELISA Kit, GFAT1 ELISA Kit, GFAT1m ELISA Kit, Gfpt ELISA Kit, CMSTA1 ELISA Kit, GFAT 1 ELISA Kit, GFPT ELISA Kit, GFPT1L ELISA Kit, MSLG ELISA Kit, glutamine--fructose-6-phosphate transaminase 1 ELISA Kit, glutamine--fructose-6-phosphate transaminase 1 S homeolog ELISA Kit, glutamine fructose-6-phosphate transaminase 1 ELISA Kit, GFPT1 ELISA Kit, gfpt1.S ELISA Kit, Gfpt1 ELISA Kit

Gene ID: 2673

UniProt: Q06210

Pathways: ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process