TMED9 ELISA Kit (Transmembrane Emp24 Protein Transport Domain Containing 9) ELISA Kit
TMED9
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant
Catalog No. ABIN6951710
$734.80
Plus shipping costs $45.00
96 tests
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Shipping to:
United States
Delivery in 21 to 31 Business Days
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- Target
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human TMED9 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Characteristics
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Expiry Date
- 6 months
-
- Target
- Alternative Name
- TMED9
- Synonyms
- GMP25, HSGP25L2G, p25, 2400003B06Rik, RGD1307627, sb:cb294, wu:fi23g02, zgc:110394, zgc:110656, Xp24a2, Xp24alpha2, tmed9, transmembrane p24 trafficking protein 9, transmembrane emp24 protein transport domain containing 9, transmembrane p24 trafficking protein 9 S homeolog, TMED9, Tmed9, tmed9, tmed9.S
- Gene ID
- 54732
- UniProt
- Q9BVK6
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