RPRD1B ELISA Kit

Catalog No. ABIN6951711

Product Details RPRD1B ELISA Kit

Target: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human RPRD1B ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for RPRD1B

Target: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Alternative Name: RPRD1B (RPRD1B Products)

Synonyms: fb23h08 ELISA Kit, zgc:55687 ELISA Kit, wu:fb23h08 ELISA Kit, rprd1ba ELISA Kit, MGC130705 ELISA Kit, C13H20ORF77 ELISA Kit, C20orf77 ELISA Kit, CREPT ELISA Kit, NET60 ELISA Kit, dJ1057B20.2 ELISA Kit, 2610304G08Rik ELISA Kit, 2810446G03Rik ELISA Kit, Crept ELISA Kit, RGD1304782 ELISA Kit, regulation of nuclear pre-mRNA domain containing 1B ELISA Kit, regulation of nuclear pre-mRNA domain containing 1B L homeolog ELISA Kit, rprd1b ELISA Kit, rprd1b.L ELISA Kit, RPRD1B ELISA Kit, Rprd1b ELISA Kit

Gene ID: 58490

UniProt: Q9NQG5