ATG10 ELISA Kit

Catalog No. ABIN6951722

Product Details ATG10 ELISA Kit

Target: ATG10 (Autophagy Related 10 (ATG10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATG10 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATG10

Target: ATG10 (Autophagy Related 10 (ATG10))

Alternative Name: ATG10 (ATG10 Products)

Synonyms: APG10 ELISA Kit, APG10L ELISA Kit, pp12616 ELISA Kit, Atg10 ELISA Kit, ATG10 ELISA Kit, MGC123341 ELISA Kit, im:7153729 ELISA Kit, zgc:123341 ELISA Kit, ATATG10 ELISA Kit, autophagy 10 ELISA Kit, DDBDRAFT_0190076 ELISA Kit, DDBDRAFT_0252608 ELISA Kit, DDB_0190076 ELISA Kit, DDB_0252608 ELISA Kit, 5330424L23Rik ELISA Kit, 5430428K15Rik ELISA Kit, AI852123 ELISA Kit, Agp10 ELISA Kit, Apg10l ELISA Kit, Apg10p ELISA Kit, Atg10l ELISA Kit, autophagy related 10 ELISA Kit, autophagy-related 10 ELISA Kit, AuTophaGy (yeast Atg homolog) ELISA Kit, ATG10 autophagy related 10 homolog (S. cerevisiae) ELISA Kit, autophagocytosis-associated family protein ELISA Kit, E2-like conjugating enzyme ELISA Kit, hypothetical protein ELISA Kit, autophagy protein 10 ELISA Kit, autophagy-related protein 10 ELISA Kit, ATG10 ELISA Kit, Atg10 ELISA Kit, atg-10 ELISA Kit, atg10 ELISA Kit, CAALFM_C302930WA ELISA Kit, APG10 ELISA Kit

Gene ID: 83734

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy