Tyrosinase-Related Protein 1 ELISA Kit

Catalog No. ABIN6951748

Product Details Tyrosinase-Related Protein 1 ELISA Kit

Target: Tyrosinase-Related Protein 1 (TYRP1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TYRP1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: TYRP1 (TYRP1 Products)

Synonyms: CAS2 ELISA Kit, CATB ELISA Kit, GP75 ELISA Kit, OCA3 ELISA Kit, TRP ELISA Kit, TRP1 ELISA Kit, TYRP ELISA Kit, b-PROTEIN ELISA Kit, LOC397853 ELISA Kit, Trypsin ELISA Kit, hm:zeh0659 ELISA Kit, tyrp1 ELISA Kit, zgc:100893 ELISA Kit, B ELISA Kit, TYRP1 ELISA Kit, tyrp-1 ELISA Kit, TRP-1 ELISA Kit, Tyrp ELISA Kit, b ELISA Kit, brown ELISA Kit, isa ELISA Kit, tyrosinase related protein 1 ELISA Kit, protease, serine 1 L homeolog ELISA Kit, tyrosinase-related protein 1b ELISA Kit, tyrosinase-related protein 1 ELISA Kit, tyrosinase-related protein 1 L homeolog ELISA Kit, tyrosinase-related protein 1a ELISA Kit, TYRP1 ELISA Kit, prss1.L ELISA Kit, tyrp1b ELISA Kit, Tyrp1 ELISA Kit, tyrp1.L ELISA Kit, tyrp1 ELISA Kit, LOC100136490 ELISA Kit, tyrp-1 ELISA Kit, tyrp1a ELISA Kit

Gene ID: 7306

UniProt: P17643