SMYD2A ELISA Kit

Catalog No. ABIN6951751

Product Details SMYD2A ELISA Kit

Target: SMYD2A (SET and MYND Domain Containing 2a (SMYD2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SMYD2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for SMYD2A

Target: SMYD2A (SET and MYND Domain Containing 2a (SMYD2A))

Alternative Name: SMYD2 (SMYD2A Products)

Synonyms: HSKM-B ELISA Kit, KMT3C ELISA Kit, ZMYND14 ELISA Kit, 1110020E07Rik ELISA Kit, 4930402C15 ELISA Kit, Zmynd14 ELISA Kit, smyd2-a ELISA Kit, im:7146196 ELISA Kit, smyd2 ELISA Kit, wu:fc02f02 ELISA Kit, zgc:110553 ELISA Kit, si:dkey-121j17.3 ELISA Kit, zgc:136609 ELISA Kit, SET and MYND domain containing 2 ELISA Kit, SET and MYND domain containing 2 S homeolog ELISA Kit, SET and MYND domain containing 2a ELISA Kit, SET and MYND domain containing 2b ELISA Kit, SMYD2 ELISA Kit, Smyd2 ELISA Kit, smyd2.S ELISA Kit, smyd2a ELISA Kit, smyd2b ELISA Kit

Gene ID: 56950

UniProt: Q9NRG4