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EDAR ELISA Kit

EDAR Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951754
  • Target See all EDAR ELISA Kits
    EDAR (Ectodysplasin A Receptor (EDAR))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human EDAR ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all EDAR ELISA Kits
    EDAR (Ectodysplasin A Receptor (EDAR))
    Alternative Name
    EDAR (EDAR Products)
    Synonyms
    rs3 ELISA Kit, edar ELISA Kit, EDAR ELISA Kit, MGC88893 ELISA Kit, DL ELISA Kit, ECTD10A ELISA Kit, ECTD10B ELISA Kit, ED1R ELISA Kit, ED3 ELISA Kit, ED5 ELISA Kit, EDA-A1R ELISA Kit, EDA1R ELISA Kit, EDA3 ELISA Kit, HRM1 ELISA Kit, dl ELISA Kit, RGD1561714 ELISA Kit, ectodysplasin A receptor ELISA Kit, ectodysplasin-A receptor ELISA Kit, edar ELISA Kit, EDAR ELISA Kit, Edar ELISA Kit
    Gene ID
    10913
    UniProt
    Q9UNE0
    Pathways
    Tube Formation, Ubiquitin Proteasome Pathway
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