UCK2 ELISA Kit

Catalog No. ABIN6960383

Product Details UCK2 ELISA Kit

Target: UCK2 (Uridine-Cytidine Kinase 2 (UCK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of UCK2 in human tissue homogenates, cell lysates, cell culture supernates.

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Uridine Cytidine Kinase 2 (UCK2)

Sensitivity: 0.056 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Reagent Preparation:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 40 ng/mL. Firstly dilute the stock solution to 10 ng/mL and the diluted standard serves as the highest standard (10 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage: 4 °C/-20 °C

Storage Comment:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Expiry Date: 6 months

Target Details for UCK2

Target: UCK2 (Uridine-Cytidine Kinase 2 (UCK2))

Alternative Name: Uridine Cytidine Kinase 2 (UCK2) (UCK2 Products)

Synonyms: TSA903 ELISA Kit, UK ELISA Kit, UMPK ELISA Kit, AA407809 ELISA Kit, AI481316 ELISA Kit, AU018180 ELISA Kit, AU020720 ELISA Kit, UMK ELISA Kit, Umpk ELISA Kit, wu:fa20g04 ELISA Kit, zgc:56174 ELISA Kit, UCK2 ELISA Kit, DDBDRAFT_0202355 ELISA Kit, DDBDRAFT_0216233 ELISA Kit, DDB_0202355 ELISA Kit, DDB_0216233 ELISA Kit, DDBDRAFT_0167798 ELISA Kit, DDBDRAFT_0230208 ELISA Kit, DDB_0167798 ELISA Kit, DDB_0230208 ELISA Kit, UCK 2-A ELISA Kit, umpk ELISA Kit, wu:fk91a01 ELISA Kit, zgc:66240 ELISA Kit, uridine-cytidine kinase 2 ELISA Kit, uridine-cytidine kinase 2b ELISA Kit, microRNA 3658 ELISA Kit, uridine-cytidine kinase 2 S homeolog ELISA Kit, uridine kinase ELISA Kit, Uridine kinase ELISA Kit, toxin secretion ABC transporter ATP-binding protein ELISA Kit, uridine-cytidine kinase 2a ELISA Kit, UCK2 ELISA Kit, Uck2 ELISA Kit, uck2b ELISA Kit, MIR3658 ELISA Kit, uck2.S ELISA Kit, LOC692983 ELISA Kit, PTRG_08949 ELISA Kit, udkA ELISA Kit, udkB ELISA Kit, Mrub_2364 ELISA Kit, Mesil_2200 ELISA Kit, Spirs_2239 ELISA Kit, Tmar_0146 ELISA Kit, Bache_3005 ELISA Kit, Celal_2781 ELISA Kit, Deima_0461 ELISA Kit, Odosp_0439 ELISA Kit, Bacsa_1376 ELISA Kit, Celly_0243 ELISA Kit, Weevi_1813 ELISA Kit, Marky_0973 ELISA Kit, Spico_0967 ELISA Kit, Poras_0295 ELISA Kit, Theth_1915 ELISA Kit, uck2 ELISA Kit, XCC1480 ELISA Kit, uck2a ELISA Kit

Pathways: Ribonucleoside Biosynthetic Process, Feeding Behaviour