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14-3-3 zeta ELISA Kit

YWHAZ Reactivity: Human, Rat, Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN6960600
  • Target See all 14-3-3 zeta (YWHAZ) ELISA Kits
    14-3-3 zeta (YWHAZ)
    Reactivity
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.31 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.31 ng/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of YWHAz in tissue homogenates, cell lysates. Due to the 99.6% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)
    Sensitivity
    0.113 ng/mL
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Top Product
    Discover our top product YWHAZ ELISA Kit
  • Comment

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Sample Volume
    100 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 20 ng/mL. Prepare 7 tubes containing 0.5 mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Sample Preparation
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Storage
    4 °C/-20 °C
    Storage Comment
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    .
    Expiry Date
    6 months
  • Target See all 14-3-3 zeta (YWHAZ) ELISA Kits
    14-3-3 zeta (YWHAZ)
    Alternative Name
    /Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) (YWHAZ Products)
    Synonyms
    14-3-3-zeta ELISA Kit, KCIP-1 ELISA Kit, YWHAD ELISA Kit, 14-3-3zeta ELISA Kit, Ywhaz ELISA Kit, ACYPI003154 ELISA Kit, 14-3-3z ELISA Kit, kcip-1 ELISA Kit, ywhaq ELISA Kit, 1433z ELISA Kit, ywhaz ELISA Kit, ywhazb ELISA Kit, 1110013I11Rik ELISA Kit, AI596267 ELISA Kit, AL022924 ELISA Kit, AU020854 ELISA Kit, ywhaza ELISA Kit, fb14h09 ELISA Kit, wu:fb05g08 ELISA Kit, wu:fb14h09 ELISA Kit, ywhai ELISA Kit, zgc:55807 ELISA Kit, 14-3-3 ELISA Kit, 14-3-3 zeta ELISA Kit, 14-3-3ZETA ELISA Kit, 14-3-3leo ELISA Kit, 2G1 ELISA Kit, 4-3-3 zeta ELISA Kit, 5.11 ELISA Kit, 549 ELISA Kit, BEST:GH05075 ELISA Kit, CG17870 ELISA Kit, D14-3-3 ELISA Kit, D14-3-3zeta ELISA Kit, Dmel\\CG17870 ELISA Kit, K ELISA Kit, LEO ELISA Kit, Leo ELISA Kit, PAR-5 ELISA Kit, PAR5 ELISA Kit, Par-5 ELISA Kit, d14-3-3zeta ELISA Kit, l(2)07103 ELISA Kit, l(2)46CFe ELISA Kit, l(2)46Ee ELISA Kit, leo ELISA Kit, par-5 ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta ELISA Kit, 14-3-3 protein zeta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta L homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta S homeolog ELISA Kit, 14-3-3 protein zeta/delta pseudogene ELISA Kit, CG17870 gene product from transcript CG17870-RE ELISA Kit, YWHAZ ELISA Kit, 14-3-3zeta ELISA Kit, ywhaz ELISA Kit, 1433z ELISA Kit, ywhaz.L ELISA Kit, Ywhaz ELISA Kit, ywhaz.S ELISA Kit, LOC100855903 ELISA Kit
    Pathways
    Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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