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Human FcRn binding Kit (TR-FRET)

TR-FRET Reactivity: Human

The kit is suitable for the detection of FcRn inhibitors. (Human FcRn binding Kit (TR-FRET))

Inhibition Assay of interaction of Human FcRn and Human IgG1 antibody by Human IgG standard in a homogeneous (no wash) TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) competition assay, with a typical IC50 of 20. (Human FcRn binding Kit (TR-FRET))

The kit has been used to detect different subclasses of Human IgG Fc proteins, which exhibit different IC50 results as expected. (Human FcRn binding Kit (TR-FRET))

The kit has been used to detect different subclasses of Human IgG, which exhibit different IC50 results as expected. (Human FcRn binding Kit (TR-FRET))

The kit has been used to detect the binding activity between Human FcRn and Human IgG standard under different pH. (Human FcRn binding Kit (TR-FRET))

The half-lives of these 3 monoclonal antibodies currently in clinical use generally correlate with the binding affinity to FcRn. (Human FcRn binding Kit (TR-FRET))

6 images

Catalog No. ABIN7580958

$991.54

Plus shipping costs $50.00

Shipping to: United States

Delivery in 2 to 4 Business Days

Quick Overview for Human FcRn binding Kit (TR-FRET) (ABIN7580958)

Target

FcRn Inhibitors

Reactivity

Human

Application

Time-resolved Fluorescence Energy Transfer (TR-FRET)

Detection Range

0.24 μg/mL-1000 μg/mL

Minimum Detection Limit

0.24 μg/mL

Purpose

The Human FcRn binding Kit is based on TR-FRET technology to measure the interaction between human FcRn and antibody drug candidates or FcRn inhibitors.

Characteristics

The Human FcRn binding Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to measure the interaction between human FcRn and antibody drug candidates or FcRn inhibitors. It is designed to facilitate the half-life evaluation of antibody drug candidates, high-throughput screening of FcRn inhibitors within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of antibody drugs to bind to FcRn.

Components

Human FCRN & B2M Heterodimer Protein Europium-chelate
FA labeled human IgG antibody
Human IgG standard
Sample Dilution Buffer
Detection Buffer
Comment
- Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
- Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
- Simple and fast operation - No complicated washing steps, significantly reducing time.
- High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
- Accurate and reliable results - High sensitivity with minimal matrix effects.
- High throughput capability - Supports 500 tests, ideal for high-throughput screening.
- Fast completion - Results in just 1 hour.
Sample Volume

10 μL

Assay Time

1 h

Protocol

This Human FcRn binding Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use the mixture of biotinylated FcRn and Europium-chelate labeled streptavidin as the donor, FA labeled Human IgG1 antibody as the acceptor.
In the absence of FcRn-binding components, the donor and acceptor are in close proximity due to the binding of FcRn and FA-labeled Human IgG1 antibody. The donor emits a 620 nm signal upon excitation, which is absorbed by the acceptor, resulting in a 665 nm emission.
In the presence of FcRn-binding components, they interfere with the donor-acceptor interaction, preventing FRET from occurring.

Restrictions

For Research Use only
Storage

4 °C

Storage Comment

Unopened kit should be stored at 2℃-8℃ upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Target

FcRn Inhibitors

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