IL12B ELISA Kit

Catalog No. ABIN7826838

Mouse IL12B ELISA Kit, Colorimetric assay for quantification of Mouse IL12B.

Quick Overview for IL12B ELISA Kit (ABIN7826838)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 pg/mL - 50 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL12B ELISA Kit

Minimum Detection Limit: 0.78 pg/mL

Purpose: This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 p40 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Cross-Reactivity (Details): This kit recognizes Mouse IL-12 p40 in samples. No significant cross-reactivity or interference between Mouse IL-12 p40 and analogues was observed

Characteristics: Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Components:

• Capture Antibody
• Reference Standard
• Micro ELISA pre-Plate
• Biotinylated Detection Antibody
• HRP Conjugate
• Manual

Material not included:

• Ancillary Reagents (ABIN7670776)
• Microplate reader with 450 nm wavelength filter
• High-precision transfer pipette
• EP tubes
• disposable pipette tips
• Incubator capable of maintaining 37 °C
• Deionized or distilled water
• Absorbent paper
• Loading slot

Application Details

Sample Volume: 100 µL

Assay Time: 1 d

Plate: Uncoated

Protocol:

1. Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
   Discard the liquid, add 200 µL ELISA Plate Blocking Buffer to each well. Incubate for 60 min at 37°C
2. Discard the liquid, add 100 µL standard or sample to the wells. Incubate for 90 min at 37°C
3. Discard the liquid, immediately add 100 µL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
4. Aspirate and wash the plate 3 times
5. Add 100 µL HRP conjugate working solution. Incubate foe 30 min at 37°C. Aspirate and wash the plate for 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37°C
7. Add 50 µL Stop Solution
8. Read the plate at 450 nm immediately. Calculation of the results

Assay Precision: Both intra-CV and inter-CV are < 10%

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Store all unopend components at -20°C for up to 12 months.

Expiry Date: 12 months

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL-12 p40

Background: IL12B, IL-12B, CLMF2, NKSF2, p40, IL-12/IL-23 p40

UniProt: P43432

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation