PRAS40 ELISA Kit

Catalog No. ABIN7830405

The Human PRAS40 ELISA Kit (ABIN7830405) is a Colorimetric ELISA Kit designed to quantify Human PRAS40.

Quick Overview for PRAS40 ELISA Kit (ABIN7830405)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PRAS40 ELISA Kit

Purpose: AKT1S1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AKT1S1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1

Background:

Synonyms: Lobe|MGC2865|PRAS40

Gene Description: AKT1 substrate 1 (proline-rich)

Gene ID: 84335

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect