SAE1 ELISA Kit

Catalog No. ABIN7830416

This Colorimetric ELISA kit is designed for the quantitative measurement of Human SAE1.

Quick Overview for SAE1 ELISA Kit (ABIN7830416)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details SAE1 ELISA Kit

Purpose: SAE1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SAE1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1

Background:

Synonyms: AOS1|FLJ3091|HSPC140|SUA1

Gene Description: SUMO1 activating enzyme subunit 1

Gene ID: 10055

UniProt: Q9UBE0