BIM ELISA Kit

Catalog No. ABIN7830445

Human BIM ELISA Kit, Colorimetric assay for quantification of Human BIM.

Quick Overview for BIM ELISA Kit (ABIN7830445)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details BIM ELISA Kit

Purpose: BCL2L11 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BCL2L11 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Alternative Name: BCL2L11

Background:

Synonyms: BAM|BIM|BIM-alpha6|BIM-beta6|BIM-beta7|BOD|BimEL|BimL

Gene Description: BCL2-like 11 (apoptosis facilitator)

Gene ID: 10018

UniProt: O43521

Pathways: PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity