Calnexin ELISA Kit
Quick Overview for Calnexin ELISA Kit (ABIN7830459)
Target
See all Calnexin (CANX) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- CANX (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CANX expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Calnexin (CANX) ELISA Kit
CANX Reactivity: Human Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Calnexin (CANX) ELISA KitCANX Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Calnexin (CANX) ELISA KitCANX Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- Calnexin (CANX)
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Alternative Name
- CANX
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Background
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Synonyms: CNX|FLJ26570|IP90|P90
Gene Description: calnexin
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Gene ID
- 821
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UniProt
- P27824
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Pathways
- MAPK Signaling, Thyroid Hormone Synthesis
Target See all Calnexin (CANX) ELISA Kits
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