CAMK2A ELISA Kit

Catalog No. ABIN7830462

The Human CAMK2A ELISA Kit (ABIN7830462) is a Colorimetric ELISA Kit designed to quantify Human CAMK2A.

Quick Overview for CAMK2A ELISA Kit (ABIN7830462)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CAMK2A ELISA Kit

Purpose: CAMK2A/CAMK2D (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CAMK2A/CAMK2D expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CAMK2A

Background:

Synonyms: CAMKA|KIAA0968

Gene Description: calcium/calmodulin-dependent protein kinase II alpha

Gene ID: 815

UniProt: Q9UQM7

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction