CAMK2B ELISA Kit

Catalog No. ABIN7830463

Human CAMK2B ELISA Kit, Colorimetric assay for quantification of Human CAMK2B.

Quick Overview for CAMK2B ELISA Kit (ABIN7830463)

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CAMK2B ELISA Kit

Purpose: CAMK2B/CAMK2G/CAMK2D (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CAMK2B/CAMK2G/CAMK2D expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CAMK2B

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Alternative Name: CAMK2B

Background:

Synonyms: CAM2|CAMK2|CAMKB|MGC29528

Gene Description: calcium/calmodulin-dependent protein kinase II beta

Gene ID: 816

UniProt: Q13554

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity