CAMK4 ELISA Kit

Catalog No. ABIN7830464

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CAMK4.

Quick Overview for CAMK4 ELISA Kit (ABIN7830464)

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CAMK4 ELISA Kit

Purpose: CAMK4 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CAMK4 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CAMK4

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Alternative Name: CAMK4

Background:

Synonyms: CaMK-GR|MGC36771

Gene Description: calcium/calmodulin-dependent protein kinase IV

Gene ID: 814

UniProt: Q16566

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma