CDK5 ELISA Kit

Catalog No. ABIN7830495

The Human CDK5 ELISA Kit (ABIN7830495) is a Colorimetric ELISA Kit designed to quantify Human CDK5.

Quick Overview for CDK5 ELISA Kit (ABIN7830495)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CDK5 ELISA Kit

Purpose: CDK5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CDK5 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background:

Synonyms: PSSALRE

Gene Description: cyclin-dependent kinase 5

Gene ID: 1020

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis