CSNK2B ELISA Kit

Catalog No. ABIN7830500

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CSNK2B.

Quick Overview for CSNK2B ELISA Kit (ABIN7830500)

Target: CSNK2B (Casein Kinase 2, beta (CSNK2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CSNK2B ELISA Kit

Purpose: CSNK2B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CSNK2B expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CSNK2B

Target: CSNK2B (Casein Kinase 2, beta (CSNK2B))

Alternative Name: CSNK2B

Background:

Synonyms: CK2B|CK2N|CSK2B|G5A|MGC138222|MGC138224

Gene Description: casein kinase 2, beta polypeptide

Gene ID: 1460

UniProt: P67870

Pathways: SARS-CoV-2 Protein Interactome