Connexin 43/GJA1 ELISA Kit

Catalog No. ABIN7830519

The Human Connexin 43/GJA1 ELISA Kit (ABIN7830519) is a Colorimetric ELISA Kit designed to quantify Human Connexin 43/GJA1.

Quick Overview for Connexin 43/GJA1 ELISA Kit (ABIN7830519)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Connexin 43/GJA1 ELISA Kit

Purpose: GJA1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GJA1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1

Background:

Synonyms: CX43|DFNB38|GJAL|ODDD

Gene Description: gap junction protein, alpha 1, 43 kDa

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization