CREB1 ELISA Kit
Quick Overview for CREB1 ELISA Kit (ABIN7830525)
Target
See all CREB1 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- CREB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CREB1 expression in cultured cells.
-
Analytical Method
- Qualitative
-
Cross-Reactivity
- Mouse, Rat
-
-
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA Kit
CREB1 Reactivity: Human pSer133 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reactivity: Mouse Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 pmol/mL - 20 pmol/mL Cell Culture Supernatant, Plasma, Serum
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reactivity: Human phosphorylated Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
cAMP Responsive Element Binding Protein 1 (CREB1) ELISA KitCREB1 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 pmol/mL - 20 pmol/mL Plasma, Serum, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Uncoated
-
Protocol
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- Store the kit at 4°C.
-
-
- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
-
Alternative Name
- CREB1
-
Background
-
Synonyms: CREB|MGC9284
Gene Description: cAMP responsive element binding protein 1
-
Gene ID
- 1385
-
UniProt
- P16220
-
Pathways
- TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
Target See all CREB1 ELISA Kits
-
