Cyclin A1 ELISA Kit

Catalog No. ABIN7830536

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Cyclin A1.

Quick Overview for Cyclin A1 ELISA Kit (ABIN7830536)

Target: Cyclin A1 (CCNA1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Cyclin A1 ELISA Kit

Purpose: CCNA1/CCNA2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCNA1/CCNA2 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Cyclin A1

Target: Cyclin A1 (CCNA1)

Alternative Name: CCNA1

Background:

Synonyms: -

Gene Description: cyclin A1

Gene ID: 8900

UniProt: P78396

Pathways: Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA