Cyclin E1 ELISA Kit

Catalog No. ABIN7830542

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Cyclin E1.

Quick Overview for Cyclin E1 ELISA Kit (ABIN7830542)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Cyclin E1 ELISA Kit

Purpose: CCNE1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCNE1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1

Background:

Synonyms: CCNE

Gene Description: cyclin E1

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases