Desmin ELISA Kit
Quick Overview for Desmin ELISA Kit (ABIN7830556)
Target
See all Desmin (DES) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- DES (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of DES expression in cultured cells.
-
Analytical Method
- Qualitative
-
Cross-Reactivity
- Mouse, Rat
-
-
Desmin (DES) ELISA Kit
DES Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Desmin (DES) ELISA KitDES Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Desmin (DES) ELISA KitDES Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Uncoated
-
Protocol
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- Store the kit at 4°C.
-
-
- Desmin (DES)
-
Alternative Name
- DES
-
Background
-
Synonyms: CMD1I|CSM1|CSM2|FLJ12025|FLJ39719|FLJ41013|FLJ41793
Gene Description: desmin
-
Gene ID
- 1674
-
UniProt
- P17661
Target See all Desmin (DES) ELISA Kits
-
