PRKDC ELISA Kit

Catalog No. ABIN7830559

Human PRKDC ELISA Kit, Colorimetric assay for quantification of Human PRKDC.

Quick Overview for PRKDC ELISA Kit (ABIN7830559)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PRKDC ELISA Kit

Purpose: PRKDC (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKDC expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: PRKDC

Background:

Synonyms: DNA-PKcs|DNAPK|DNPK1|HYRC|HYRC1|XRCC7|p350

Gene Description: protein kinase, DNA-activated, catalytic polypeptide

Gene ID: 5591

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response