ErbB2/Her2 ELISA Kit
Quick Overview for ErbB2/Her2 ELISA Kit (ABIN7830573)
Target
See all ErbB2/Her2 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- ERBB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ERBB2 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA Kit
ErbB2/Her2 Reactivity: Human Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Rat Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Human AA 23-652 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Human Soluble Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum
Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) ELISA KitErbB2/Her2 Reactivity: Human Colorimetric Sandwich ELISA 781 pg/mL - 50000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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Alternative Name
- ERBB2
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Background
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Synonyms: CD340|HER-2|HER-2/neu|HER2|NEU|NGL|TKR1
Gene Description: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
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Gene ID
- 2064
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UniProt
- P04626
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
Target See all ErbB2/Her2 ELISA Kits
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