H2AFX ELISA Kit

Catalog No. ABIN7830577

Human H2AFX ELISA Kit, Colorimetric assay for quantification of Human H2AFX.

Quick Overview for H2AFX ELISA Kit (ABIN7830577)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details H2AFX ELISA Kit

Purpose: H2AFX (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of H2AFX expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX

Background:

Synonyms: H2A.X|H2A/X|H2AX

Gene Description: H2A histone family, member X

Gene ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus