HSPE1 ELISA Kit

Catalog No. ABIN7830589

Human HSPE1 ELISA Kit, Colorimetric assay for quantification of Human HSPE1.

Quick Overview for HSPE1 ELISA Kit (ABIN7830589)

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details HSPE1 ELISA Kit

Purpose: HSPE1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HSPE1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for HSPE1

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Alternative Name: HSPE1

Background:

Synonyms: CPN10|GROES|HSP10

Gene Description: heat shock 10 kDa protein 1 (chaperonin 10)

Gene ID: 3336

UniProt: P61604

Pathways: Positive Regulation of Endopeptidase Activity