IGFBP3 ELISA Kit
Quick Overview for IGFBP3 ELISA Kit (ABIN7830596)
Target
See all IGFBP3 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- IGFBP3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IGFBP3 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse
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Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
IGFBP3 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Dog Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Mouse Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Chicken Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA KitIGFBP3 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
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Alternative Name
- IGFBP3
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Background
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Synonyms: BP-53|IBP3
Gene Description: insulin-like growth factor binding protein 3
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Gene ID
- 3486
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UniProt
- P17936
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Pathways
- Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
Target See all IGFBP3 ELISA Kits
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