IL13 Receptor alpha 1 ELISA Kit

Catalog No. ABIN7830602

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL13 Receptor alpha 1.

Quick Overview for IL13 Receptor alpha 1 ELISA Kit (ABIN7830602)

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details IL13 Receptor alpha 1 ELISA Kit

Purpose: IL13RA1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL13RA1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for IL13 Receptor alpha 1

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Alternative Name: IL13RA1

Background:

Synonyms: CD213A1|IL-13Ra|NR4

Gene Description: interleukin 13 receptor, alpha 1

Gene ID: 3597

UniProt: P78552

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response